study on the components of a nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni

by Coral Dawn Bell

Publisher: s.n.] in [Toronto

Written in English
Published: Pages: 133 Downloads: 58
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Subjects:

  • Nuclear polyhedrosis virus,
  • Trichoplusia ni

Edition Notes

  There is an NPV marketed as Fawligen that is specific to Spodoptera larvae, which are fall armyworm and beet armyworm. Another NPV product, Loopex, is specific to the cabbage looper, Trichoplusia ni. All of the NPV products will not infect other insects or arthropods, including pests and beneficial predators and parasites. The genome of the Trichoplusia ni single nucleopolyhedrovirus (TnSNPV), a group II NPV which infects the cabbage looper (T. ni), has been completely sequenced and analyzed. The TnSNPV DNA genome consists of , bp and has an overall G + C content of 39%. In this study, we describe experiments, in which Trichoplusia ni (cabbage looper) larvae were coinfected with the L1 strain of wild-typeAutographa californica nuclear polyhedrosis virus (AcMNPV) and an occ – derivative of the same virus (occ – 1).   The cabbage looper, T. ni, is susceptible to Bt toxin at a very low dose, as demonstrated by the diet overlay bioassay. The addition of mM CPT resulted in increasing in the mortality observed with or g/L of Bt WP at 25 ± % and 25 ± % to 75 ± % and ± %, respectively.

  In: Adms JR, Bonami JR (eds) Atlas of invertebrate viruses. CRC Press, Boca Raton, pp – Del Rincon-Castro MC, Ibarra JE () Genotypic divergence of three single nuclear polyhedrosis virus (SNPV) strains from the cabbage looper, Trichoplusia ni. . Search the history of over billion web pages on the Internet.   Natural Enemies. Cabbage looper is attacked by numerous natural enemies, and the effectiveness of each seems to vary spatially, temporally, and with crop environment. Most studies noted the effectiveness of wasp and tachinid parasitoids, and a nuclear polyhedrosis virus (NPV). Predation has not been well-studied except in cotton. The piggyBac transposon was originally isolated from the cabbage looper moth, Trichoplusia ni, in the s. Despite its early discovery and dissimilarity to the other DNA transposon families, the piggyBac transposon was not recognized as a member of a large transposon superfamily for a long time. Initially, the piggyBac transposon was thought to be a rare transposon.

The cabbage looper Trichoplusia ni, a common agricultural pest, was sublethally challenged with the bacterial pathogen Bacillus thuringiensis through the natural route of exposure (feeding). Within‐generation immune priming to B. thuringiensis ingestion has been demonstrated previously in T. ni . 1 Introduction. The cultured Spodoptera frugiperda (Sf9), army fallworm, and the cabbage looper Trichoplusia ni have been established as cell lines for the production of foreign proteins after infection with respective recombinant baculoviruses AcMNPV (Autographa californica nuclear polyhedrosis virus). This system is a promising tool for the overproduction and large‐scale purification of.   Transposons are mobile genetic elements that can be cut out of and inserted into DNA. They are present in most living things and make up almost half of the human genome. Transposons help to rearrange and increase the variety of DNA sequences, which can drive evolution and regulate the expression of genes. Enzymes called transposases help to move transposons, but very few genes . General Methods 4 Laboratory Studies 4 Small-Scale Field Tests 5 Large-Scale Field Tests 7 Reporting Microbial Agent Test Results 8 Annual Row Crops 12 Fiber Crops 12 Cotton 12 Boll and Square Feeders: Bollworm and Tobacco Budworm 13 Pink Bollworm 14 Foliage Feeders: Armyworms, Cabbage Looper, Cotton Leaf Worm 16 Oil Crops 16 Corn 16 Peanuts.

study on the components of a nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni by Coral Dawn Bell Download PDF EPUB FB2

Bell CD, Orlob GB. Serological studies on virions and polyhedron protein of a nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni. Virology. May 1; 78 (1)– Bramhall S, Noack N, Wu M, Loewenberg JR.

A simple colorimetric method for determination of protein. Anal Biochem. Oct 1; 31 (1)–Cited by:   Improved purification procedures were employed to isolate polyhedron protein and enveloped virus particles (virions) from a nuclear polyhedrosis virus (NPV) of the cabbage looper, Trichoplusia ni, to study the serological relationship between these two dra were digested in sodium carbonate-saline buffer, pH 11, for 1 hr at room temperature and centrifuged at Cited by: In my thesis, I examined the coevolution of cabbage loopers (Trichoplusia ni Hubner) and its associated singly-embedded nuclear polyhedrosis virus (TnSNPV).

I chose T. ni because it is a generalist herbivore of great economic importance and because it has a short generation time thus making it a good candidate for studies of : Maynard Lionel Milks. Description. The polyhedral capsid from which the virus gets its name is an extremely stable protein crystal that protects the virus in the external environment.

It dissolves in the alkaline midgut of moths and butterflies to release the virus particle and infect the larva. An example of an insect that it infects is the fall webworm. NPV was once listed by the International Committee on Group: Group I (dsDNA).

Scott HA, Young SY, 3rd, McMasters JA. Isolation and some properties of components of nuclear polyhedra from the cabbage looper, Trichoplusia ni. J Invertebr Pathol. Sep; 18 (2)– Shvedchikova NG, Tarasevich LM. Electron microscope investigation of granulosis viruses of Dendrolimus sibiricus and Agrotis segetum.

J Invertebr Pathol. The cabbage looper (Trichoplusia ni) is a medium-sized moth in the family Noctuidae, a family commonly referred to as owlet common name comes from its preferred host plants and distinctive crawling behavior.

Cruciferous vegetables, such as cabbage, bok choy, and broccoli, are its main host plant; hence, the reference to cabbage in its common name. Animal Science (General) - (LL) Animal Anatomy and Morphology (New March ) - (LL) Animal Behaviour - (LL) Animal Breeding and Genetics (Discontinued March ) - (LL) Animal Genetics - (LL) Animal Genetics and Breeding (New March ) - (LL).

Larvae of the cabbage looper, Trichoplusia ni, were infected with a nuclear polyhedrosis virus and the protein patterns of the hemolymph followed throughout the course of the disease by. chapter 39 - dual infection of the trichoplusia ni cell line with the chilo iridescent virus (civ) and autographa californica nuclear polyhedrosis virus M.

Kimura and A.H. McIntosh Pages A mutant (m) of Autographa californica nuclear polyhedrosis virus (AcMNPV) grew in Spodoptera frugiperda and Trichoplusia ni cells but did not form typical intranuclear occlusion bodies (OB.

Summary. Cultures of cell line IZD-MB from Mamestra brassicae were inoculated with nonoccluded nuclear polyhedrosis virus of Autographa californica (AcNOV).

At 1 h postinoculation (p.i.) nucleocapsids were found in the cytoplasm near nuclear pores and within the nucleoplasm. Structural studies on the polyhedral inclusion bodies, virions, and DNA of the nuclear polyhedrosis virus of the cotton bollworm Heliothis zea. 1 Coronavirus: Find the latest articles and preprints.

Cabbage loopers Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) are serious pests in greenhouses growing tomatoes, cucumbers and bell peppers. A potential microbial control, now in development, is the broad host-range virus Autographa californica multiple nucleopolyhedrovirus (AcMNPV). The relationships between the three host plants and the feeding behaviour, larval movement and.

In order to examine how an insect reacts to baculovirus challenge, we measured components of the cellular and humoral immune response of the cabbage looper Trichoplusia ni to Trichoplusia ni SNPV, a narrow-host range nucleopolyhedrovirus (NPV), over four doses and three times after pathogen challenge (18, 42 and 90 h).

Serumfree cultures of insect cells play an important role in the fields of protein engineering, medicine, and biology. In this paper, the suspension cell line QB-Tns of Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) was successfully adapted to serumfree Sf III medium and passaged for 52 generations.

The adapted QB-Tns cells grew faster. The first studies to address the nuclear import of baculovirus were performed by Summers, who infected the larvae of the cabbage looper Trichoplusia ni (Tn) with the Betabaculovirus TnGV and analyzed larvae’s midgut tissues by electron microscopy at different time post-infection [46,47].

These studies revealed TnGV nucleocapsids associated. The cabbage looper is attacked by numerous natural enemies, and the effectiveness of each seems to vary greatly. Most studies note the effectiveness of wasp and tachinid parasitoids, and a nuclear polyhedrosis virus (NPV).

Predation has not been well studied except in cotton. Faulkner, P. and Henderson, J.F. () Serial passage of a nuclear polyhedrosis virus of the cabbage looper (Trichoplusia ni) in a continuous tissue culture cell line.

Virol – CrossRef Google Scholar. Chapter Replication of Alfalfa Looper Nuclear Polyhedrosis Virus in the Trichoplusia Ni (Tn) Cell Line Chapter An Electron Microscope Study of the Sequence of Events in a Nuclear Polyhedrosis Virus Infection in Cell Culture Chapter Like most viruses, baculoviruses tend to be species or genus specific, although there are some exceptions to this rule, notably the Autographa californica nuclear polyhedrosis virus.

Much of the genetics work currently being done to improve baculovirus-based pesticides is concentrated in the area of the virus genome controlling its host range. Ignoffo, C. M.: Production and virulence of a nuclear polyhedrosis virus from larvae of Trichoplusia ni (Htibner) reared on a semisynthetic diet.

Insect Path. 6, 18– (). Google Scholar. Beaton CD, Filshie BK. Comparative ultrastructural studies of insect granulosis and nuclear polyhedrosis viruses.

J Gen Virol. May; 31 (2)– Bell CD, Orlob GB. Serological studies on virions and polyhedron protein of a nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni. Virology. May 1; 78 (1)– (). Serological studies on virions and polyhedron protein of a nuclear polyhedrosis virus of the cabbage looper, Trichoplusisa ni.

Solubilization of Trichoplusia ni granulosis virus. tion. In this study, we describe experiments, in which Tricho-plusia ni (cabbage looper) larvae were coinfected with the L1 strain of wild-type Autographa californica nuclear polyhedrosis virus (AcMNPV) and an occ– derivative of the same virus (occ–1).

Our data showed considerable persistence of the occ–. Expression and Immunological Studies of Classical Swine Fever Virus Glycoprotein E2 in the Bi-Cistronic Baculovirus/Larvae Expression System Chi-Ming WU,1 Shih-Ling HSUAN,1 Zeng-Weng CHEN,2 Tzyy-Rong JINN,3 Chienjin HUANG,2 Jiunn-Wang LIAO, 1Ter-Hsin CHEN,;2 Chih-Ming LIAO,1 Wei-Cheng LEE,1 Tzong-Yuan WU,4 and Maw-Sheng CHIEN1;y 1Graduate Institute of Veterinary.

to apply the model to assess commercially produced strains of the nuclear polyhedrosis virus of the cabbage pest Trichoplusia ni. More concretely, gypsy moth populations tend to be very patchily distributed, so that a major challenge for managers is identifying which populations need to be controlled and which are likely to collapse.

Fielding, B.C.; Davison, S. The characterization and phylogenetic relationship of the Trichoplusia ni single capsid nuclear polyhedrosis virus polyhedrin gene. Virus Genes19. In this study we ask whether parasite productivity is positively correlated with host food availability (a proxy of host condition) in the virus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and one of its natural hosts, the cabbage looper moth (Trichoplusia ni, Hübner, Lepidoptera: Noctuidae).

Generalist insects should possess the ability to rank potential host plants for the suitability of their offspring. The decision to oviposit on a host plant that is inferior for larval development could have significant consequences for their offspring.

Host plant quality can affect larval development and survival directly via nutrients and/or defensive chemicals, as well as indirectly by. The cabbage looper (Trichoplusia ni) is a moth in the family Noctuidae, a family commonly referred to as owlet common name comes from its preferred host plants and distinctive crawling behavior.

Cruciferous vegetables, such as cabbage, bok choy, and broccoli, are its main host plant; hence, the reference to cabbage in its common name. [1]. An insect cell line has been established and characterized, derived from embryonic tissue (BTI-TN-5B, ATCC CRL ) of Trichoplusia ni (cabbage looper).

The line is susceptible to various baculoviruses, including TnSNPV and AcMNPV, and may be used to replicate such viruses for use as insecticides or otherwise.Cabbage looper populations experienced up to 40% infection by virus in the same studies.

Mass reared viruses have been successfully applied in limited areas as microbial insecticides against pests. In trials in Maryland, releases of granulosis virus proved as effective as Bt against some caterpillars and, in trials against imported cabbageworm.PubMed:Recovery of the nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni, by coprecipitation with lactose.

PubMed:The biosynthesis of steryl glucosides in plants. PubMed:[Biosphere products of Sinapis alba. I. Rhizosphere products]. PubMed:[ON THE ANTHOCYANINS IN RED CABBAGE.